This deliverable provides information on the protocols for surface functionalization and synthesis of DNA on wafers.
The photolithographic synthesis of DNA on glass is a straightforward process that can be executed through methodologies that have been established and optimised at the University of Vienna – UniVie. In the context of BioAssembler, the challenge is to adapt these procedures for synthesis on wafers. In this regard, two aspects are critical: to define the strategy employed for functionalizing the surface to facilitate the addition of the initial nucleotide, and to ascertain the compatibility of all involved procedures and reagents in the synthesis and deprotection steps with the materials on the sensor surface.
Thus, deliverable D4.1 is associated with task 4.1 of Work Package 4 (DNA Zipcoding).
